解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::The mitochondrial MURF4 gene of T. brucei has pronounced G versus C strand bias and heterogeneously sized transcripts, characteristic of genes encoding extensively edited transcripts. We find that MURF4 transcripts of T. brucei are extensively edited throughout by the addition and deletion of numerous uridines, creati...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(90)90199-o
更新日期:1990-06-01 00:00:00
abstract::Partially edited mRNAs were selected by the polymerase chain reaction and sequenced. In the case of cytochrome b, 102 out of 106 clones displayed patterns of editing that were consistent with a strictly progressive 3' to 5' editing process, as predicted by the guide RNA model of RNA editing. In the case of cytochrome ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(90)90197-m
更新日期:1990-06-01 00:00:00
abstract::The mechanism by which MPF induces nuclear lamin disassembly and nuclear envelope breakdown during mitosis was studied in a frog egg extract in which the transition from interphase to mitosis can be induced by the addition of MPF. Bacterially expressed human nuclear lamin C, assembled in vitro into filaments, showed i...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(90)90469-u
更新日期:1990-05-18 00:00:00
abstract::Initiation of tumorigenesis in mouse skin can be accomplished by mutagenesis of the H-ras gene by treatment with chemical carcinogens. A mouse model system has been developed to study the additional genetic events that take place during tumor progression. Skin carcinomas were induced in F1 hybrid mice exhibiting restr...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(90)90523-h
更新日期:1990-05-04 00:00:00
abstract::The full-length cDNA for a transcriptional activator, DBP, that binds to the D site of the albumin promoter has been cloned. DBP belongs to a family of related transcription factors including Fos, Jun, CREB, and C/EBP, which share a conserved basic domain. However, unlike most other members of this family, DBP does no...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(90)90808-r
更新日期:1990-04-20 00:00:00
abstract::We have identified a gene, SRV2, mutations of which alleviate stress sensitivity in strains carrying an activated RAS gene. Epistasis analysis suggests that the gene affects accumulation of cAMP in the cell. Direct assays of cAMP accumulation indicate that mutations of the gene diminish the rate of in vivo production ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(90)90813-t
更新日期:1990-04-20 00:00:00
abstract::Cadherins are a group of homophilic intercellular adhesion molecules; each member of this family exhibits binding specificity. Here, we attempted to map the sites for the specificities of these molecules by analyzing adhesives selectivities of the cells that express chimeric and point-mutated E- and P-cadherin. The re...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(90)90222-z
更新日期:1990-04-06 00:00:00
abstract::During fruiting body development, the product of the csgA gene is necessary for cellular aggregation, for spore differentiation, and for gene expression that is initiated after 6 hr of starvation. From nascent wild-type fruiting bodies we have purified a polypeptide of 17 kd called C-factor, which, at approximately 1 ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(90)90211-v
更新日期:1990-04-06 00:00:00
abstract::In Drosophila melanogaster males, the sex chromosomes pair during meiosis in the centric X heterochromatin and at the base of the short arm of the Y (YS), in the vicinity of the nucleolus organizers. X chromosomes deficient for the pairing region segregate randomly from the Y. In this report we show that a single ribo...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(90)90215-z
更新日期:1990-04-06 00:00:00
abstract::A gene, bcl-3, is found on chromosome 19 adjacent to the breakpoints in the translocation t(14;19)(q32;q13.1), which occurs in some cases of chronic lymphocytic leukemia. Sequence analysis of the human bcl-3 gene predicts a protein containing seven tandem copies of the SWI6/cdc10 motif. This motif was previously ident...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(90)90347-h
更新日期:1990-03-23 00:00:00
abstract::Expression of human immunodeficiency virus type 1 structural proteins requires both the viral Rev trans-activator and its cis-acting RNA target sequence, the Rev response element (RRE). The RRE has been mapped to a conserved region of the HIV-1 env gene and is predicted to form a complex, highly stable RNA stem-loop s...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(90)90670-a
更新日期:1990-02-23 00:00:00
abstract::We have identified a novel octamer binding factor (Oct-3) in P19 embryonal carcinoma cells. Oct-3, which recognizes the typical octamer motif (ATTTGCAT) as well as the AT-rich sequence TTAAAATTCA, is present in P19 stem cells but disappears when the cells are induced to differentiate by retinoic acid (RA). Cloned cDNA...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(90)90597-8
更新日期:1990-02-09 00:00:00
abstract::Novel, approximately 90 bp intervening sequences (IVs) were discovered within the 23S rRNA genes of S. typhimurium and S. arizonae. These non-rRNA sequences are transcribed and then excised during rRNA maturation. The rRNA fragments that result from the excision of the extra sequences are not religated. This results i...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(90)90592-3
更新日期:1990-02-09 00:00:00
abstract::The S. pombe nuc2+ gene is required for mitotic chromosome disjunction. Its mutation arrests mitosis at the metaphase. The gene product is present in the nuclear scaffold-like fraction. The nuc2+ protein contains a domain, separated from ten 34 amino acid repeat segments, that is capable of binding AT-rich DNA in vitr...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(90)90746-2
更新日期:1990-01-26 00:00:00
abstract::E. coli RecA protein promotes homologous pairing and reciprocal strand exchange reactions between duplex DNA molecules in vitro. Reaction intermediates contain Holliday junctions that are driven along the DNA at a maximal rate approaching 1000 bases per minute. T4 endonuclease VII cleaves Holliday junctions in vitro, ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(90)90747-3
更新日期:1990-01-26 00:00:00
abstract::We have investigated whether histone H1 is present on active Balbiani ring genes in the salivary glands of Chironomus tentans using immunoelectron microscopy. The genes were studied in two activity states: maximally activated genes with a fully extended template and repressed genes in a 30 nm fiber conformation. Histo...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(90)90717-s
更新日期:1990-01-12 00:00:00
abstract::Fos protein can trans-activate AP-1-dependent gene expression and trans-repress the c-fos promoter. Although we find that trans-repression is enhanced by coexpression of c-Jun, it does not require any of the AP-1 or ATF sites in the mouse c-fos promoter. A major target for repression is the serum response element (SRE...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(89)90756-3
更新日期:1989-12-22 00:00:00
abstract::While the nuclear lamin proteins (A, B, and C) assemble specifically at the surface of the nuclear membrane, their sequences do not reveal stretches of hydrophobic amino acids that might explain their association with the nuclear membranes. However, the A and B lamin proteins possess Ras-like C-terminal CaaX sequence ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(89)90753-8
更新日期:1989-12-22 00:00:00
abstract::Two precursor cell subpopulations have been isolated from the spleen cells of nonimmune mice. The major B cell subpopulation binds high levels of the J11D monoclonal antibody and, upon T cell-dependent antigenic stimulation, gives rise to primary antibody-forming cell clones but not secondary B cells. A minority of th...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(89)90761-7
更新日期:1989-12-22 00:00:00
abstract::The transcriptional induction of heat shock genes is mediated by the heat shock transcription factor (HSF). We have investigated the oligomerization state of HSF from S. cerevisiae and find that it forms a trimer in solution and when bound to DNA. Trimerization is mediated by a region of HSF that, like the leucine zip...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(89)90604-1
更新日期:1989-12-01 00:00:00
abstract::The minimal DNA sequence required for the formation of a stable complex with Drosophila heat shock factor (HSF) in vitro is an inverted repeat of a 5 bp recognition unit, -GAA-. Surprisingly, both permutations of this 5 bp unit, head-to-head and tail-to-tail, bind to HSF with similar affinity and with striking 2-fold ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(89)90603-x
更新日期:1989-12-01 00:00:00
abstract::The CD4 lymphocyte surface glycoprotein and the lck tyrosine protein kinase p56lck are found as a complex in T lymphocytes. We have defined the domains in both proteins that are responsible for this interaction by coexpressing hybrid and deleted forms of the two proteins in HeLa cells. We have found that the unique 32...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(89)90008-1
更新日期:1989-11-17 00:00:00
abstract::Subcellular compartments in which folding and assembly of proteins occur seem to have a set of PCB proteins capable of mediating these and related processes, such as translocation across membranes. When a domain of a polypeptide chain emerges from a ribosome during synthesis or from the distal side of a membrane durin...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/0092-8674(89)90005-6
更新日期:1989-11-17 00:00:00
abstract::I have identified an activity in crude HeLa cell extracts that satisfies the requirements for a human telomere terminal transferase or telomerase. It catalyzes the addition of a 6 nucleotide repeating pattern to oligonucleotide primers containing human or nonhuman telomeric repeat sequences. Direct sequence analyses o...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(89)90035-4
更新日期:1989-11-03 00:00:00
abstract::We report the identification and characterization of a class of IS10 transposase mutants that carry out only some of the steps required for transposition. These mutants were identified among transposition-defective mutants as a specific subclass that retains the wild-type ability to induce SOS functions in the presenc...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(89)90299-7
更新日期:1989-10-20 00:00:00
abstract::We have located the distal boundary of the tyrosine kinase domain of the EGF receptor and have identified a distinct sequence in the C' terminus required for EGF-dependent receptor internalization, leading to receptor down-regulation and degradation. Within this receptor domain, an 18 amino acid highly negatively char...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(89)90867-2
更新日期:1989-10-06 00:00:00
abstract::Calmodulin is implicated as the primary transducer of the calcium signal in pancreatic beta cells, where it is present at very high concentrations. We have produced three lines of transgenic mice carrying a calmodulin minigene regulated by the rat insulin II promoter. Immunohistochemistry and hybridization analyses in...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(89)90505-9
更新日期:1989-09-22 00:00:00
abstract::Embryonic inductions appear to be mediated by the concerted action of different inducing factors that modulate one another's activity. Such modulation is likely to reflect interactions between the signal transduction pathways through which the inducing factors act. We tested this idea for the induction of neural tissu...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(89)90099-8
更新日期:1989-08-25 00:00:00
abstract::Mouse macrophages and lymphocytes express two distinct isoforms of a single class of Fc receptor for IgG. The macrophage isoform (FcRII-B2) is identical to the lymphocyte isoform (FcRII-B1) except for an inframe insertion in the cytoplasmic tail of FcRII-B1 that increases its length from 47 to 94 amino acids. To deter...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(89)90846-5
更新日期:1989-07-28 00:00:00
abstract::The molecular basis for membrane association of pp60v-src, the transforming protein of Rous sarcoma virus, was investigated in a cell-free system. Newly synthesized pp60v-src polypeptide, produced by in vitro translation of src mRNA, rapidly bound to plasma membranes. Binding was saturable and dependent on the presenc...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(89)90842-8
更新日期:1989-07-28 00:00:00
abstract::The HIV-1 Tat protein is a potent trans-activator essential for virus replication. We reported previously that HIV-1 Tat peptides containing residues 37-48 (mainly region II), a possible activating region, and residues 49-57 (region III), a nuclear targeting and putative nucleic acid binding region, possess minimal bu...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(89)90417-0
更新日期:1989-07-14 00:00:00
abstract::In the mouse, the zona pellucida (ZP) glycoprotein ZP3 both binds intact sperm and induces acrosomal exocytosis. The subsequent signaling pathway(s) is still uncertain, but Gi-like proteins have been implicated. By analogy with other signal transduction mechanisms, we examined anti-phosphotyrosine antibody reactivity ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(89)90049-4
更新日期:1989-06-30 00:00:00
abstract::The specificity of target gene activation by steroid receptors is encoded within a small, cysteine-rich domain that is believed to form two zinc-coordinated fingers. Here we show that the ability of glucocorticoid and estrogen receptors to discriminate between their closely related response elements resides in the two...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(89)90050-0
更新日期:1989-06-30 00:00:00
abstract::DNA fragments containing the silencers that flank the mating type genes at HML alpha are shown to bind specifically to the nuclear scaffold of yeast. The scaffold proteins are solubilized with urea and then renatured to form a soluble extract which allows reconstitution of sequence-specific DNA loops. At the silent ma...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(89)90788-5
更新日期:1989-06-02 00:00:00
abstract::An injection of aphidicolin into early Drosophila embryos inhibits DNA synthesis and nuclear division, while centrosome replication and many other aspects of the mitotic cycle continue. If aphidicolin is injected at nuclear cycle 7-8, the normal migration of nuclei to the embryo cortex is completely inhibited. In most...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(89)90130-x
更新日期:1989-05-19 00:00:00
abstract::Translation of ribosomal proteins in the alpha operon of E. coli is repressed by one of the encoded proteins, S4; it specifically recognizes an RNA fragment containing the translational initiation site for the first gene in the operon. RNA structure mapping experiments have suggested a pseudoknot structure for the S4 ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(89)90123-2
更新日期:1989-05-19 00:00:00
abstract::The effects of beta-estradiol (estrogen; a minor component of yeast cells) on S. cerevisiae cells in the G0 and G1 phases were examined. Results showed that estrogen stimulated the recovery of growth from G0 arrest induced by nutrient limitation or ts mutation of cdc35 (adenylate cyclase) in the early G1 phase, and in...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(89)90136-0
更新日期:1989-05-19 00:00:00
abstract::Despite the obvious importance of epithelial stem cells in tissue homeostasis and tumorigenesis, little is known about their specific location or biological characteristics. Using 3H-thymidine labeling, we have identified a subpopulation of corneal epithelial basal cells, located in the peripheral cornea in a region c...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(89)90958-6
更新日期:1989-04-21 00:00:00
abstract::The yeast tropomyosin gene, designated TPM1, is present in a single copy per haploid genome and encodes a protein with a predicted molecular weight of 23.5 kd. The protein sequence is homologous to higher cell tropomyosins, including the characteristic hydrophobic-hydrophilic pseudoheptapeptide repeats. Indirect immun...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(89)90961-6
更新日期:1989-04-21 00:00:00
abstract::Two cDNAs were isolated whose dimerized products bind specifically to a DNA sequence, kappa E2, located in the immunoglobulin kappa chain enhancer. Both cDNAs share a region of extensive identity to the Drosophila daughterless gene and obvious similarity to a segment in three myc proteins, MyoD, and members of the Dro...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(89)90682-x
更新日期:1989-03-10 00:00:00